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Study on the Hepatoprotective Effect of Oxalis coriniculata L. and Related Mechanism by Regulating Oxidative Stress and TLR-2/NF-κB Signaling Pathway

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【作者】 Ya GAOChun CHENKefeng ZHANGRiming WEI

【Author】 Ya GAO;Chun CHEN;Kefeng ZHANG;Riming WEI;Guilin Medical University;

【通讯作者】 Riming WEI;

【机构】 Guilin Medical University

【摘要】 [Objectives] This study aimed to explore the protective effect of Oxalis coriniculata L. on rats with acute liver injury induced by carbon tetrachloride(CCl4) and related mechanism by regulating oxidative stress and the TLR-2 TLR-2/NF-κB signaling pathway. [Methods] A total of 48 female rats were randomly and evenly divided into normal group, model group, silymarin group(0.12 g/kg), and high(16 g/kg), middle(8 g/kg) and low-dose(4 g/kg) O. coriniculata L. groups. The rats in the groups were intragastrically administered with 5 mL/kg of corresponding drugs(equal-volume distilled water for normal group and control group), respectively. The administration was conducted twice a day, for 10 consecutive days. After 2 h of the last administration, the rats in all the groups except the normal group were intraperitoneally injected with 12% carbon tetrachloride(CCl4) olive oil solution(5 mL/kg), respectively to establish liver injury rat models. After 16 h, the eyeball blood of the rats was collected, and their liver tissues were collected for preparation of HE sections. The biochemical indicators detected included aspartate aminotransferase(AST), alanine aminotransferase(ALT), total superoxide dismutase(T-SOD) and glutathione peroxidase(GSH-Px) activity and malondialdehyde(MDA) content in the serum. The contents of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and interleukin-6(IL-6) in the serum were detected by ELISA. The expression of Toll-like receptor-2(TLR-2) and nuclear factor-κB(NF-κB) in liver tissue was detected using Western blotting. The pathological changes of liver were observed under light microscope. [Results] Compared with the normal group, the ALT, AST activity and MDA, IL-1β, IL-6, TNF-α levels in rat serum significantly increased(P<0.01), the GSH-Px, T-SOD activity in rat serum significantly decreased(P<0.01), and the expression of TLR-2 and NF-κB in liver tissue was up-regulated(P<0.01) in the model group. Compared with the model group, the ALT, AST activity and MDA, IL-1β, IL-6 and TNF-α levels in rat serum reduced(P<0.05, P<0.01), the GSH-Px and T-SOD activity in rat serum increased(P<0.05, P<0.01), and the expression of TLR-2 and NF-κB in liver tissue was down-regulated(P<0.05, P<0.01) in the O. coriniculata L. administration groups. Pathological sections show that O. coriniculata L. had an improving effect on rats with acute liver injury induced by CCl4. [Conclusions] O. coriniculata L. has a good protective effect on rats with acute liver injury induced by CCl4. Its mechanism may be related to inhibition of oxidative stress, inhibition of inflammatory response and regulation of the TLR-2/NF-κB signaling pathway.

【基金】 Supported by Bagui Scholar Program of Guangxi(Gui Cai Jiao Han[2017]No.143)
【所属期刊栏目】 Pharmacological and Toxicological Analyses (2019年06期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2019.06.014
  • 【分类号】R285.5
  • 【下载频次】3
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