节点文献

Antimicrobial Mechanism of Saponin from Sapindus mukorossi against Escherichia coli

免费订阅

【作者】 Faling LEILei XIAOChunyan YAOZengli WANG

【Author】 Faling LEI;Lei XIAO;Chunyan YAO;Zengli WANG;Infinitus (China) Company Ltd.;Zhejiang Academy of Medical Sciences;Healthy Star Bio-Tech R&D Ltd.;

【通讯作者】 Chunyan YAO;

【机构】 Infinitus (China) Company Ltd.Zhejiang Academy of Medical SciencesHealthy Star Bio-Tech R&D Ltd.

【摘要】 [Objectives] To investigate the antimicrobial activity and mechanism for saponin from Sapindus mukorossi against Escherichia coli. [Methods] E. coli was used as the test bacteria, and the antimicrobial effect of Sapindus saponin was studied by the minimum inhibitory concentration method. The antimicrobial mechanism of Sapindus saponin was studied in terms of growth curves, membrane potential, activity of cells, cell surface morphology and cell internal structure. [Results] The results indicate that Sapindus saponin can inhibit the growth of E. coli, the minimum inhibitory concentration is 5 mg/mL and has a significant inhibitory effect on the growth of E. coli. After treated with Sapindus saponin, the membrane potential of E. coli increased by 16.7, 17.3 and 19.1 times after exposition to 0.5, 1, 2 MIC Sapindus saponin respectively. PI staining results show that cell viability decreased and permeability of cell membranes increased. The results of SEM and TEM further corroborate the membrane damage, the cell was damaged after exposition to Sapindus saponin. [Conclusions] The antimicrobial mechanism of Sapindus saponin were to changed membrane potential of cell, and damage the cell membrane structures.

【所属期刊栏目】 Pharmacological and Toxicological Analyses (2019年06期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2019.06.015
  • 【分类号】R285
  • 【下载频次】2
节点文献中: 

本文链接的文献网络图示:

浏览历史:
下载历史: