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Establishment of Specific Chromatogram of Spica Prunellae Formula Granules Based on Standard Decoction

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【作者】 Xinwei NONGMei YANGXiongmei HUANGHuilian DENGChan WANGJian ZHOU

【Author】 Xinwei NONG;Mei YANG;Xiongmei HUANG;Huilian DENG;Chan WANG;Jian ZHOU;Pura Pharm (Nanning) Pharmaceutical Co.,Ltd.;

【通讯作者】 Jian ZHOU;

【机构】 Pura Pharm (Nanning) Pharmaceutical Co.,Ltd.

【摘要】 [Objectives] This study aimed to establish HPLC chromatograms of decoction pieces, standard decoction and formula granules of Spica Prunellae. [Methods] The chromatographic conditions were as follows: column, SHISEIDO CAPCELL PAK C18 MGII column(4.6 mm×250 mm, 5 μm); mobile phase, acetonitrile-0.1% phosphoric acid; gradient elution; detection wavelength, 280 nm; flow rate, 1.0 mL/min; and column temperature, 30 ℃. The correlation between the decoction pieces, standard decoction and formula granules of Spica Prunellae was analyzed by specific chromatograms. [Results] The fingerprint chromatograms of decoction pieces, standard decoction and formula granules of Spica Prunellae showed five common peaks, with good correlation. Among the five common peaks, four of them were tanshinol, protocatechuic acid, caffeic acid and rosmarinic acid. [Conclusions] The main chemical constituents of decoction pieces, standard decoction and formula granules of Spica Prunellae are basically the same. The HPLC specific chromatogram established can be used for the quality control of Spica Prunellae formula granules.

【基金】 Supported by Scientific Research and Technology Development Project of Nanning City(20173158-5)
【所属期刊栏目】 Processing of Traditional Chinese Medicine and Quality Control (2019年06期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2019.06.019
  • 【分类号】R286.0
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