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Effects of Paeonol on Expression of Type Ⅰ and Type Ⅲ Collagen of High Glucose Induced Proliferation of Cardiac Fibroblasts

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【作者】 Dandan WUXiaohui ZHOU

【Author】 Dandan WU;Xiaohui ZHOU;Chengde Medical College;

【通讯作者】 Xiaohui ZHOU;

【机构】 Chengde Medical College

【摘要】 [Objectives] To explore the effects of paeonol on the inhibition of myocardial fibrosis in high glucose induced cardiac fibroblasts(CFs). [Methods] Differential adherence method was used to culture the primary CFs of neonatal rats(passage culture); CCK-8 method was used to detect the cell proliferation; MTT assay was used to screen the safe concentration of paeonol; the 2 nd to 3 rd generation CFs were randomly divided into normal group(5.5 mmol/L, expressed in C), high glucose group(30 mmol/L, expressed in HG), paeonol low dose group(Pae-L, 17.5 mg/L), and medium dose paeonol group(Pae-M, 35 mg/L), paeonol high dose group(Pae-H, 70 mg/L); Western Blot method was used to detect the expression of Col-I and Col-III protein. [Results] The extraction of CFs from primary neonatal rats was successful; high glucose(30 mmol/L) induction had a significant proliferation effect on CFs; compared with the normal group, the expressions of COI-I and Col-III protein were increased in the high glucose group(P<0.05); compared with the high glucose group, the expression of COI-I protein was decreased in each treatment group(P<0.05, P<0.01), and the decrease was most significant in the high dose group(P<0.01); the expression of COI-III in the high dose group was decreased and it was statistically significant(P<0.05). [Conclusions] Paeonol significantly inhibited the proliferation of high glucose induced CFs in neonatal rats. This experiment is intended to provide a new experimental basis for the prevention and treatment of diabetic cardiomyopathy(DCM).

【所属期刊栏目】 Clinical Application (2019年06期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2019.06.024
  • 【分类号】R285
  • 【下载频次】3
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