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Optimization of Extraction Process and Antioxidant Activity of Polysaccharide in Embelia parviflora Wall. by RSM

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【作者】 Wenli LIXianxian LIUXiaolian LIANGYong CHENDanqing PANGZhouyan HUANGQian HANWenjun LIULiangni CHEN

【Author】 Wenli LI;Xianxian LIU;Xiaolian LIANG;Yong CHEN;Danqing PANG;Zhouyan HUANG;Qian HAN;Wenjun LIU;Liangni CHEN;College of Pharmacy, Guangxi University of Chinese Medicine;

【通讯作者】 Yong CHEN;

【机构】 College of Pharmacy, Guangxi University of Chinese Medicine

【摘要】 [Objectives] To optimize the extraction process of polysaccharide in Embelia parviflora Wall. by response surface methodology, and to study the antioxidant activity in vitro of polysaccharide in E. parviflora, so as to provide a basis for the further development and utilization of E. parviflora. [Methods] The solid-to-liquid ratio, extraction temperature, extraction time and extraction times were used as single factors to investigate the effects. On this basis, the response surface methodology(RSM) was used to optimize the extraction process of polysaccharide in E. parviflora. DPPH and ABTS free radicals were used to investigate the antioxidant capacity of polysaccharide in E. parviflora. [Results] The optimal solid-to-liquid ratio for extraction of polysaccharide from E. parviflora was 32∶1(mL/g), extraction temperature was 77 ℃, and extraction time was 36 min. The IC50 values of DPPH and ABTS were 0.04 and 0.03 mg/mL, respectively. [Conclusions] The optimized extraction process of polysaccharide in E. parviflora is stable, the extraction rate is high, and has strong antioxidant activity. It is expected to provide a reference for the industrial extraction of polysaccharide from E. parviflora.

【基金】 Supported by National Natural Science Foundation of China(81660701);Natural Science Foundation of Guangxi(2016GXNSFAA380148);Scientific Research Innovation Program of Guangxi University of Chinese Medicine(YJSZ201712);Program of Key Laboratory for Purification and Quality Analysis of TCM Extraction in Guangxi Universities(Gui Jiao Ke Yan[2014]No.6)
【所属期刊栏目】 Processing of Traditional Chinese Medicine and Quality Control (2020年03期)
  • 【DOI】10.19600/j.cnki.issn2152-3924.2020.03.010
  • 【分类号】R284;R285
  • 【下载频次】12
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