Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole（Cynoglossus semilaevis）

Tian-Zi WANGAi SUNNa WANGZhong-Kai CUISong-Lin CHENZhen-Xia SHA

Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences Function Laboratory for Marine Fisheries Science and Food Production Processes,National Lab for Ocean Science and Technology

摘要：An astroglial cell line was established from the brain of half smooth tongue sole（Cynoglossus semilaevis）and was designated as CSAC.CSAC shows the morphological homogeneity of epithelial cells.The cell identity was tested by the presence of glial fibrillary acidic protein（GFAP）,which was revealed by RT-PCR and immunofluorescence.The cell line was optimally maintained at 24 °C in minimum essential medium supplemented with HEPES,antibiotics,20%fetal bovine serum,2-Mercaptoethanol（2-Me） and basic fibroblast growth factor.Chromosome analysis revealed that the CSAC cells maintained a normal diploid chromosome number（2n=42）.The fluorescent signals were observed in CSAC after the cells were transfected with green fluorescent protein（GFP） reporter plasmids.The CSAC cell line may serve as a valuable tool for studies on the potential functions of fish astroglial cells.

基金资助：

supported by grants from the National Natural Science Foundation of China（31130057）； the State863 High-Technology R&D Project of China（2012AA10A408）； the Taishan Scholar Project Fund of Shandong Province of China； the Special Scientific Research Funds for the Central Non-profit Institutes,Chinese Academy of Fishery Sciences（2013A0402）；