节点文献

Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques(Macaca leonina)

免费订阅

【作者】 Xiao-Liang ZHANGJia-Hao SONGWei PANGYong-Tang ZHENG

【Author】 Xiao-Liang ZHANG;Jia-Hao SONG;Wei PANG;Yong-Tang ZHENG;Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences;Faculty of Life Science and Technology, Kunming University of Science and Technology;Institute of Health Sciences, Anhui University;Kunming Primate Research Center of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences;

【机构】 Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of SciencesFaculty of Life Science and Technology, Kunming University of Science and TechnologyInstitute of Health Sciences, Anhui UniversityKunming Primate Research Center of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences

【摘要】 Northern pig-tailed macaques(NPMs, Macaca leonina) are susceptible to HIV-1 infection largely due to the loss of HIV-1-restricting factor TRIM5α. However, great impediments still exist in the persistent replication of HIV-1 in vivo, suggesting some viral restriction factors are reserved in this host.The APOBEC3 proteins have demonstrated a capacity to restrict HIV-1 replication, but their inhibitory effects in NPMs remain elusive. In this study, we cloned the NPMA3A-A3H genes, and determined by BLAST searching that their coding sequences(CDSs) showed 99% identity to the corresponding counterparts from rhesus and southern pig-tailed macaques. We further analyzed the anti-HIV-1 activities of the A3A-A3H genes, and found that A3G and A3F had the greatest anti-HIV-1 activity compared with that of other members. The results of this study indicate that A3G and A3F might play critical roles in limiting HIV-1 replication in NPMs in vivo. Furthermore, this research provides valuable information for the optimization of monkey models of HIV-1 infection.

【基金】 supported by the National Special Science Research Program of China(2012CBA01305);National Natural Science Foundation of China(81172876;81471620;81273251;81571606;U0832601);National Science and Technology Major Project(2014ZX10005-002-006);Knowledge Innovation Program of CAS(KJZD-EW-L10-02);Yunnan Applicative and Basic Research Program(2014FB181)
【所属期刊栏目】 Articles (2016年04期)
  • 【DOI】10.13918/j.issn.2095-8137.2016.4.246
  • 【分类号】R512.91;R-332
  • 【被引频次】2
  • 【下载频次】8
节点文献中: 

本文链接的文献网络图示:

浏览历史:
下载历史: